XB-IMG-144201
Xenbase Image ID: 144201
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Fig. 1. Xnr3 injection induced an abnormal neural pattern in
Xenopus embryos. (A,B) Superficial phenotype of 2-day tadpole
after Xnr3 injection. (A) An uninjected embryo. (B) An embryo injected
with 100 pg of Xnr3 mRNA into the ventral animal pole (VAP)
region at the 4-cell stage. (C-N) Whole-mount in situ hybridization
(WISH) of stage-18 embryos. Normal embryos (C,E,G,I,K,M) and
embryos injected with 40 pg of Xnr3 mRNA into the VAP region
at the 4-cell stage (D,F,H,J,L,N) showing the spatial expression of
xOtx2 (C, D), Xrx1 (E, F), CG1 (G, H), krox20, En2, and xBF1 (I, J),
slug (K, L), and Sox2 (M, N). In (I,J), krox20/En2 expression extended
laterally (black arrow) and curved anteriorly (white arrow). In (K,L),
200 pg of lacZ was also injected. Arrows in (L) show the anterior
elongation of the slug expression domain. (O) Quantitative levels of
brain marker genes measured by RT-PCR. Experiments were carried
out with the anterior region dissected from stage-18 embryos. 0
pg (lane 1), 40 pg (lane 2), or 100 pg (lane 3) of Xnr3 mRNA were
injected into the VAP of 4-cell embryos, and the expressions of xOtx2
(lane 1), Xrx1 (column 2), xCG1 (column 3), krox20 (column 4), En2
(column 5), slug (column 6), Sox2 (column 7) and ODC (column 8)
were analyzed. (P-U) Expression patterns of neural markers in late
neurula after treatment of the embryos with RA. Expression of slug
(P-R), krox20, and xOtx2 (S-U). The embryos were treated with 5 x
10-7 M RA (Q,T) or 10-6 M RA (R,U). Image published in: Morita M et al. (2013) Copyright © 2013. Reproduced with permission of the Publisher, University of the Basque Country Press.
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