Xenbase Image ID: 144203

Fig. 3. The mature region of Xnr3 contributed to abnormal neural patterning. (B-M) Spatial expression of neural marker genes was observed by WISH. 750 pg of pXtnr3 (C,G,K), 100 pg of mXtnr3 (D,H,L), or 40 pg of Xnr3 mRNA (E,I,M) was injected into the VAP region of 4-cell embryos. pXtnr3 and mXtnr3 indicate the pro-region and the mature-region, respectively, of Xtnr3. Embryos were examined with probes for krox20 and En2 (B-E), Xslug (F-I), xOtx2 (L-M). pXtnr3 did not affect the expression patterns, whereas mXtnr3 injection induced patterns similar to those observed after injection of Xnr3. Arrow indicates the change in expression pattern. (N) Synergistic enhancement of the activity of mXtnr3 by pXtnr3 expression. Spatial expression of En2/krox20 at stage 18 was assessed by WISH, and the results are expressed in a bar graph. The severity of the effect neural is categorized into three classes: Class 1, Class 2 and Class 3 are represented by white, orange and red, respectively. Image published in: Morita M et al. (2013) Copyright © 2013. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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