Xenbase Image ID: 144204

Fig. 4. Xnr3 injection specifically affected the shape of cells at the epidermal-neural boundary. (A) The experimental procedure is illustrated in a schematic diagram. Alexa 568 and Xnr3 mRNA were injected into the DAP and the VAP region, respectively. At stage 18, the epidermal-neural boundary regions of the anterior end of neural plate and lateral neural plate were dissected and stained with phalloidin-Alexa488. (B-G) Double-fluorescent labeling of the anterior (B-D) or lateral (E-G) region of a normal embryo. The Alexa568 dextran staining pattern (B,E), the phalloidin staining pattern (C,F), and a merged image (D,G) are shown. The Alexa568-positive region approximately shows the presumptive neural plate. (H-M) Doublestained patterns in the anterior region (H-J) and lateral region (K-M) of an Xnr3-injected embryo. The Alexa568 dextran staining pattern (H, K), the phalloidin staining pattern (I, L), and a merged image (J, M) are shown. Image published in: Morita M et al. (2013) Copyright © 2013. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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