Xenbase Image ID: 1443

Fig. 3. Depletion of kermit 2/XGIPC specifically inhibits Xenopus eye development. (A-D) Depletion of kermit 2 does not affect the expression of dorsal mesoderm markers chordin and goosecoid. Embryos are viewed from the vegetal side and dorsal is towards the top. Control morpholino or kermit 2 morpholino (40 ng) was injected into two dorsal animal blastomeres of four-cell embryos. Embryos were fixed at the gastrula stage (stage 10) and whole-mount in situ hybridization was performed for chordin (A,B) and goosecoid (C,D). (E-L) Depletion of kermit 2 specifically reduces marker gene expression within the presumptive eye field in stage 20 embryos. Embryos are viewed from the anterior side with dorsal towards the top. Control or kermit 2 morpholino was injected into one dorsal animal blastomere of four-cell/eight-cell embryos with 500 pg of mRNA for nuclear β-galactosidase. Embryos were fixed at stage 20 andβ -galactosidase activity was measured in situ (red) followed by whole-mount in situ hybridization for Bf1 (E,F), Otx2 (G,H), Pax6 (I,J) and Xrx (K,L). Bf1 expression was not affected by depletion of kermit 2 (F). Expression of Otx2 (H) and Pax6 (J) were reduced only within the presumptive eyeforming region (red arrowheads) in embryos injected with kermit 2 morpholino. The expression of the eye marker Xrx was also inhibited (L red arrowhead). (M-P) Depletion of kermit 2 does not reduce Xrx (N) or Pax6 (P) expression in early neurula stage embryos (stage 14). (Q-S) Kermit 2 mRNA restored Xrx expression in kermit 2-depleted embryos. Red arrowhead in R indicates strongly reduced Xrx expression within presumptive eye domain in kermit 2-depleted embryo; red arrow in S indicates recovered Xrx expression in embryo co-injected with kermit 2 morpholino and kermit 2 mRNA. Image published in: Wu J et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10 marginal zone dorsal marginal zone foxg1.L bf1, foxg1b, LOC108700624, XBF-1, XBf1 X. laevis Throughout NF stage 20 pre-chordal neural plate neural plate otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 20 anterior neural tube eye primordium optic field pax6.S an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Sometime during NF stage 14 to NF stage 20 anterior neural tube eye primordium pre-chordal neural plate rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Sometime during NF stage 14 to NF stage 20 eye primordium gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10 upper blastopore lip dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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