Xenbase Image ID: 144356

Figure 5. Inhibiting Sox2 Expression Has a Similar Effect on Eye Development as Inhibiting Xfz5 Expression (A and B) Reduced eye size with Sox2 MO injection at the 8-cell stage. (C) Sox2 MO injection causes reduction in anti-phosphohistone H3 (HP3) staining within the optic vesicle at stage 23/24. Uninjected side is the control. n = 8 embryos. (D) Retinal clone size is reduced by the Sox2 MO. GFP RNA was coinjected, and the number of GFP-labeled cells was counted in six central retinal sections for each embryo (n = 9 embryos). In (C) and (D), error bars represent SEM; *p < 0.001 by Student’s t test. Sox2 MO does not affect the intensity of Sox2 (E), Xfz5 (F), Rx (G), Pax6 (H), Six6 (I), and Chx10 (J) expression within the optic vesicle. En expression (arrowhead in [G]) is unchanged. The Sox2 MO suppresses the retinal expression of Xash3 (K) and Xdelta-1 (L). Injected side is on the right (arrow). Image published in: Van Raay TJ et al. (2005) Copyright © 2005. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page