Xenbase Image ID: 144357

Figure 6. TCF/Lef-Dependent Transcription Is Active in the Developing Eye and Is Required for Normal Eye Development The TOP:dGFP transgene (A) is expressed in the developing eye (arrowhead) at stage 20 (B), and stage 23 (C), as revealed by in situ hybridization. The midbrain/hindbrain boundary is marked by En expression (arrow in [B]) or reporter expression (arrow in [C]). (D) Rx promoter driving ΔN-Tcf3 tagged with GFP (Rx:ΔN-Tcf3-GFP). (E) GFP antibody staining reveals transgene expression in the developing eye (stage 23). (F) Disrupted eye development in transgenic embryos (stage 41). (G) Sections through the same embryo reveal loss of neural retina tissue, but remnants of RPE and lens (L). Section is stained with the nuclear dye Hoechst (blue). Image published in: Van Raay TJ et al. (2005) Copyright © 2005. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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