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Figure 8. Inhibiting Xfz5 Expression in Retinal Progenitors Biases Cells to Adopt the Mu¨ller Glial Cell Fate (A) Expression of dnXfz5 in retinal progenitors by in vivo lipofection caused a 4-fold increase in the representation of Mu¨ller glia as compared to GFP controls. n = 1706 cells from 26 embryos for GFP, n = 402 cells from 9 embryos for dnXfz5. (B) Retinal section (stage 41) showing a GFP-labeled cell (green) expressing dnXfz5 that is colabeled using antibodies for the Mu¨ller glial cell marker CRALBP (red). (C) Expression of dnLRP6 in retinal progenitors by in vivo lipofection also caused a significant increase in the representation of Mu¨ller glia as compared to GFP controls. n = 391 cells from 6 embryos for GFP, n = 252 cells from 5 embryos for dnLRP6. For (A) and (C), the percent representation of each cell type was calculated as a weighted average, and error bars represent SEM; **p < 0.001, *p < 0.05 by Student’s t test. G, ganglion cells; H, horizontal cells; A, amacrine cells; B, bipolar cells; PR, photoreceptor cells; M, Mu¨ller glial cells. Image published in: Van Raay TJ et al. (2005) Copyright © 2005. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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