Xenbase Image ID: 144396

Figure 3. Integrin Dominant Negative Disrupts Bipolar Cell Intercalations (A–D) Confocal images of dextran-labeled deep cells were collected from (A and C) mesoderm and (B and D) neural tissues of Keller sandwich explants undergoing extension. Cells take on normal bipolar morphologies in both (A) mesoderm and (B) neural tissues of explants expressing HA. In explants made from embryos expressing HAβ1, there are no bipolar cells in either (C) mesoderm or (D) neural tissues. The red arrows indicate the anterior-posterior axis; anterior is toward the top of the figure in panels (A)–(D). (E and F) In 18 hr explants that express HA, the notochord cells have converged toward the midline ([E], nuclei indicated by yellow arrowheads), producing a narrow structure ([E], red arrow). In HAβ1-expressing explants of a similar age, the notochord is broader ([F], red arrow), and the location of the notochord cell nuclei ([F], yellow arrowheads) indicates that cells have not converged toward the midline. Grayscale images (E) and (F) have been inverted to clarify cell boundaries. Image published in: Marsden M and DeSimone DW (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page