Xenbase Image ID: 144397

Figure 4. FN Matrix Is Required for Elongate Cell Morphologies in Explants Undergoing Convergence and Extension Movements (A–E) Keller sandwiches made by combining DMZs expressing HA and HAβ1. Elongate cell phenotypes ([A], dextran-labeled cells) are observed in the half of the explant that assembles FN ([B], FN revealed with 32F polyclonal Ab). In the half of the explant that does not assemble an FN matrix, cells take on a disorganized rounded phenotype, but a boundary between notochord and somitic mesoderm is still evident ([A], red arrowheads). (A) and (B) were obtained from 10 hr explants and represent a saggital section through an area similar to that indicated by the red box in Figure 2K. (C–E) Keller sandwiches were also made from a combination of DMZs expressing HA and HAβ1 colabeled with green and red dextrans, respectively. The HA-expressing mesoderm cells take on normal bipolar phenotypes ([C], grayscale image of green channel shown in [D]), unless surrounded by HAβ1-expressing cells ([C], red arrowheads). HAβ1-expressing cells have a rounded appearance ([D], red dextran; [E] is grayscale image of red channel shown in [D]), unless surrounded by HA-expressing cells ([E], red arrowheads). Image published in: Marsden M and DeSimone DW (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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