Xenbase Image ID: 144399

Figure 6. Cell Sorting of Dissociated DMZ Cells Is Altered by Expression of the Integrin Dominant-Negative Construct Aggregates were made from HA- and/or HAβ1-injected DMZ-dissociated deep cell layers and were allowed to sort for 3 hr at room temperature. (A) When HA-expressing green and red dextran-labeled cells are combined, they aggregate but do not sort. (B) Red and green dextran-labeled cells expressing HAβ1 also aggregate but do not sort. (C) Sorting also does not occur when green and red cells expressing HA are treated with the C-cadherin-activating antibody AA5. (D and E) When HA-expressing cells (red) are combined with HAβ1 cells (green), cell sorting is evident after (D) 1 hr, and, by (E) 3 hr, the two cell populations are segregated; aggregates of red cells are shed at the periphery (data not shown). FN matrix can be detected in aggregates after 3 hr of association with cells expressing HA ([E], inset). (F) Activation of C-cadherin by mAb AA5 inhibits the exclusion of HA (red) cells from the mixed aggregates. Three individual assays were performed on three separate spawnings for a total of nine aggregates of each type; representative samples are shown. Image published in: Marsden M and DeSimone DW (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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