XB-IMG-144402
Xenbase Image ID: 144402
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Figure S2. Anti-FN Antibodies Inhibit Convergent
Extension in Open Face Explants
(A–F) (A, C, and E) Bright-field views of 16 hr
open-faced explants. (B, D, and F) The same
explants at higher magnification under epifluorescence
to reveal FN fibrils. Incubation of
open-faced explants in a mAb that blocks
FN fibril assembly gives variable results with
regard to both the (A and C) extent of extension
and the appearance of (B and D) FN fibrils.
A paucity of fibrils (B) is associated with
a lack of extension in these explants (A). Intermediate
extension is noted in some explants
incubated with 1F7 (C), and in these cases,
this correlates well with the appearance of
some fibrils (D). In all explants incubated with
control mAb 4H2, extension was robust and,
correspondingly, an extensive fibrillar network
was detected in the extending tissue
(F). Antibody treatments were as indicated in
each panel (4H2, control anti-FN mAb; 1F7,
FN-blocking mAb; Rb anti-FN, rabbit anti-
Xenopus FN polyclonal #32). Image published in: Marsden M and DeSimone DW (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |