XB-IMG-144523
Xenbase Image ID: 144523
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Figure 1. Manipulation of Dishevelled Localization
(A) Dvl monomers associate via their N-terminal
DIX domains to form oligomers that
are found in both cytoplasmic and cell-membrane
populations (A#).
(B) Endogenous Dvl monomers will oligomerize
with membrane-tethered Dvl-caax
such that the wild-type Dvl is sequestered at
the cell membrane (B#).
(C) Endogenous Dvl monomers will oligomerize
with mitochondrial-localized Xdsh-
MA, and the wild-type Dvl will be sequestered
in the cytoplasm, away from the cell
periphery (C#).
(D) Localization of Xdsh-GFP in a normal
Xenopus animal cap.
(E) Higher-magnification view of Xdsh-GFP
(green) localization in normal animal cap; cell
membrane is indicated by memRFP (E#, red).
(F) Wild-type Xdsh-GFP (green) is translocated
to the cell membrane by expression of
unlabeled Dvl-KM-caax.
(G) Higher-magnification view of a cell expressing
wild-type Xdsh-GFP (green) and
unlabeled Dvl-KM-caax; cell membrane is indicated
by memRFP (G#, red).
(H) Wild-type Xdsh-GFP (green) is translocated
to clusters in the cytoplasm by expression
of unlabeled Xdsh-MA.
(I) Higher-magnification view of a cell expressing
wild-type Xdsh-GFP (green) and
unlabeled Dvl-KM-caax; cell membrane is indicated
by memRFP (I#, red). Image published in: Park TJ et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |