XB-IMG-144524
Xenbase Image ID: 144524
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Figure 2. Removal of Dishevelled from the
Cell Membrane in the DMZ Blocks Convergent
Extension by Disrupting Cell Shape
and Cell Polarity
(A) Membrane localization of Xdsh-GFP in
the DMZ of a normal embryo. Cell membrane
can be visualized by memRFP (A#). Colocalization
is apparent in the merged image (A$).
(B) Xdsh-GFP is entirely sequestered in the
cytoplasm in the DMZ of embryos expressing
Xdsh-MA. (B#) shows coexpressed
memRFP. (B$) shows a merged image.
(C) Control embryo showing clone of cells
from injection of fluorescent dextran (green)
at the 256-cell stage. Labeled clone is more
obvious in the fluorescent view in panel (C#).
(D) Labeled clones at late gastrulation have
undergone significant convergence and extension
(compare to panel [C]). Fluorescent
view is in panel (D#).
(E) Confocal image of cells in the boxed region
of panel (D#). Because of mediolateral
cell intercalation, labeled cells are intermingled
with unlabeled cells. These cells are
mediolaterally elongated and aligned.
(F) Xdsh-MA-injected embryo showing a
labeled clone of cells that is not elongated
at early gastrulation (fluorescent view in
panel [F#]).
(G) Labeled clones at late gastrulation in Xdsh-MA-injected embryos have failed to elongate (fluorescent view in panel [G#]).
(H) Confocal image of cells in the boxed region of panel (G#). In embryos expressing Xdsh-MA, labeled cells are not intermingled with
unlabeled cells. These cells are not mediolaterally elongated and are randomly oriented (see Figure S3). Image published in: Park TJ et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |