XB-IMG-144546
Xenbase Image ID: 144546
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Figure S2 (related to Figures 2 and 3). Domain structure and mutant
construction of Kdm2a and β-Catenin, and the effect of Kdm2a on the β-Catenin
mutants.
(A) Kdm2a domain structure and mutant construction.
(B) Summary of β-Catenin mutant construction and interaction affinities of different
mutants with Kdm2a. +: interaction; -: no interaction; n.t.: not tested.
(C) In a time course CHX treatment of HEK293T cells, Kdm2a transfection enhanced
the degradation of endogenous β-Catenin.
(D) Quantification of (C) in triplicate. Error bars show the SEM.
(E) Transfection of different doses of Kdm2a caused no significant change in
β-Catenin mutant that contained no NLS or was fused to an NLS.
In (C) and (E), WCL were used for IB. Image published in: Lu L et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |