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Figure S3 (related to Figure 4). Test of specificity of different antibodies for detecting the methylation of β-Catenin. (A) Detection of methylation status of immunoprecipitated β-Catenin with different antibodies, among which only the KpanMe detected a specific band. Note that the exposure time for the immunoblots for other three antibodies was much extended. (B) Detection of the non-phosphorylated β-Catenin from the proteins that were precipitated with different antibodies. Only the proteins precipitated by KpanMe and β-Catenin showed cross-reaction with the nonphosphorylated β-Catenin antibody. Immunoprecipitates were used for IB.

Image published in: Lu L et al. (2015)

Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.

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