Xenbase Image ID: 144547
Figure S3 (related to Figure 4). Test of specificity of different antibodies for
detecting the methylation of β-Catenin.
(A) Detection of methylation status of immunoprecipitated β-Catenin with different
antibodies, among which only the KpanMe detected a specific band. Note that the
exposure time for the immunoblots for other three antibodies was much extended.
(B) Detection of the non-phosphorylated β-Catenin from the proteins that were
precipitated with different antibodies. Only the proteins precipitated by KpanMe and
β-Catenin showed cross-reaction with the nonphosphorylated β-Catenin antibody.
Immunoprecipitates were used for IB.
Image published in: Lu L et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
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