Xenbase Image ID: 144605

Figure 1. Identification of XL221p20 as a Molecule that Plays a Role in Gastrulation, by Expression Cloning (A and B) Overexpression of XL221p20 causes gastrulation defects. The percentages of normal (open), short trunk (shaded), and spina bifida (solid) embryos are indicated in (B) (n = 60). (C) Mesodermal specification is unaffected by XL221p20 overexpression. The expression of goosecoid (gsc), Xenopus brachyury (Xbra), and Xenopus Wnt11 (Xwnt11) was detected by WISH at stage 11 (dorsal-vegetal view). Both control (ctrl; upper panels) and XL221p20-overexpressing (lower panels) embryos were given injections of β-gal as a lineage tracer (red). (D) XL221p20 is expressed in the axial mesoderm and neuroectoderm in gastrulae. The XL221p20-expressing region was detected by WISH at stage 11 (Da, Dd, De, and Dg), stage 13 (Db and Dh), and stage 15 (Dc, Df, and Dl). An enlarged view of the hemisection image ([Dd], square) is shown in (De). The triangle in (Df) points to the notochord. No signal was detected by the sense probe (Dg–Di). Image published in: Hyodo-Miura J et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue arfgap3.S xgap X. laevis Throughout NF stage 11 mesoderm dorsal marginal zone tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 mesoderm gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 involuted dorsal mesoderm wnt11.S Wnt11-R, WNT11-related, wnt11a, Wnt11r X. laevis Throughout NF stage 11 mesoderm arfgap3.S xgap X. laevis Throughout NF stage 13 mesoderm axial mesoderm arfgap3.S xgap X. laevis Throughout NF stage 15 notochord cranial neural crest neural crest notochordal sheath Image source: Published Larger Image Printer Friendly View

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