XB-IMG-144608
Xenbase Image ID: 144608
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Figure 4.
The Conserved C-Terminal Region, but Not the GAP Domain, Is Essential for XGAP to Control Gastrulation Cell Movements
(A) Truncated constructs of XGAP and a summary of their Mo phenotype-suppressing activity and intracellular localization. The orange box and green box indicate the GAP domain and conserved region, respectively.
(B–E) Suppression of the XGAP-Mo phenotype by coinjection of each truncated mRNA. XGAP-δN and XGAP-C, but not XGAP-N and XGAP-δC, suppressed the gastrulation-defective phenotype caused by XGAP-Mo, in a dose-dependent manner (100 pg, 200 pg, or 500 pg; n = 70). XGAP-δC appeared to be a null allele.
(F–I) Intracellular localization of each truncated construct of XGAP in the DMZ. The mRNA for each Venus-tagged construct was injected into the dorsal blastomeres at the four-cell stage at a low dose (100 pg) that induced no phenotype. XGAP-δN and XGAP-C, but not XGAP-N or XGAP-δC, accumulated at the tips of the spindle-shaped cells. XGAP-C was also detected in the nucleus. White dots indicate the cell shape. The lower panels show the intensity of Venus. The magenta bar indicates the x axis and scanning was from left to right.
Image published in: Hyodo-Miura J et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |