Xenbase Image ID: 144616

Figure S5. XGAP-DN Accelerates Cell Movement (A, B) XGAP-DN increased the distance cells moved toward a scratched field. To compare the protrusive activity of cells expressing each construct, we performed a wound-healing assay using cultured cells as an experimental model. MDCK (Madin-Darby canine kidney) cells were transfected with DNA constructs for Venus (not shown), XGAP (upper panels), XGAP-DN (middle panels), or XGAP-DC (lower panels). The cells were cultured to confluence in DMEM supplemented with 10% fetal bovine serum. The MDCK cell sheets were then gently stripped with a sterile 200-μl pipette tip, and the cell movements were monitored using a confocal microscope after the scratch (0 hour; left panels) until 2 hours later (right panels). The closed and opened triangles indicate the start and final line of the healing wound, respectively. The bar graph shows the average distance moved in 2 hours for each condition (n = 25) in (B). Image published in: Hyodo-Miura J et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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