XB-IMG-144617
Xenbase Image ID: 144617
|
Figure S6. XGAP Has GAP Activity for xARF6
(A) XGAP binds specifically to ARF6. HEK293T cells were co-transfected with XGAP-Flag
and each of the Myc-tagged ARFs (ARF1, ARF4, and ARF6) , and an immunoprecipitation
was performed. ARF1 and ARF4 seemed to degrade even though a large quantity of plasmid
was used in the transfection.
(B) XGAP binds to ARF6 through its GAP domain. Each of the truncated constructs was
co-expressed in HEK293T cells with Myc-ARF6.
(C) XGAP has GAP activity for ARF6. ARF6-myc was expressed in HeLa cells alone or with
XGAP (FL) or XGAP-DN (DN). Each cell lysate was incubated with GST-GGA3 GAT protein
[the deactivation of Myc-tagged ARF6 by XGAP was assayed as described previously
(Venkateswarlu et al., 2004) with minor modifications], then pulled down with
glutathione-Sepharose beads and detected with an anti-Myc antibody.
(D) ARF6 is expressed in the ectoderm during gastrulation. ARF6 mRNA was detected by
WISH at stage 11.5 (a, b) and stage 15 (c, d). Panels (a) and (c) show a whole embryo with a
vegetal view (dorsal is to the top) and dorsal view, respectively. Panels (b) and (d) are
hemisection (dorsal is to the right) and cross-section images, respectively. In (d), the
arrowhead indicates the notochord. Image published in: Hyodo-Miura J et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
Image source: Published Larger Image Printer Friendly View |