Xenbase Image ID: 144671

Fig. 3. Voltage-dependent potassium (Kv) subunit-like immunoreactivity in developing retinal ganglion cells (RGCs) and their axons. A: Double labelling of a stage 33/34 retina with anti-Kv1.3 (green) and mouse anti-bromodeoxyuridine (BrdU; red) antibodies shows that in the central retina Kv1.3-like immunopositive cells (arrows) are not BrdU positive. B: Double labelling (yellow) with antibodies against the rat Kv4.2 subunit and islet-1 at stage 33/34. The islet-1 antibody stains all RGCs (red), but the Kv4.2 antibody (green) labels only a subset of these islet-1–positive RGCs. C: Double labelling (yellow) of a stage 41 retina with a Muller glial marker (3CB2) and the anti- Kv4.2 antibody shows that radial cell processes in the central third of the retina are immunopositive (arrows) for both markers, whereas processes in the peripheral retina are immunopositive for the Kv4.2- like subunit only (arrowheads). By this stage the optic nerve head (onh), but not RGC somata, are Kv4.2-like immunopositive. D: Merged image of E and F shows colocalization of the Kv3.4 subunit and actin filaments in an RGC growth cone. E: Actin labelled with rhodamine phalloidin. F: The Kv3.4 antibody labels the axon and growth cone. IPL, inner plexiform layer; L, lens; OPL, outer plexiform layer; RGCL, RGC layer. Scale bar 100 m for A–C and 10 m for D–F. Image published in: Pollock NS et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue kcna3.S k(v)1.3, kv1.3 X. laevis Throughout NF stage 33 and 34 ciliary marginal zone retina kcnd3.L K(v)4.3, kv4.3, LOC108709434 X. laevis Throughout NF stage 33 and 34 retinal ganglion cell retinal ganglion cell layer retina kcnd3.L K(v)4.3, kv4.3, LOC108709434 X. laevis Throughout NF stage 41 axon optic nerve optic disc retinal outer plexiform layer retinal inner plexiform layer isl1.S islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 33 and 34 retinal ganglion cell retinal ganglion cell layer retina actb.L beta actin, beta-actin, g-actin X. laevis Sometime during NF stage 33 and 34 to NF stage 40 retinal ganglion cell axon growth cone kcnc4 hkshiiic, kshiiic, kv3.4, LOC108709468 X. tropicalis Sometime during NF stage 33 and 34 to NF stage 40 retinal ganglion cell axon growth cone Image source: Published Larger Image Printer Friendly View

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