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Figure 6. Dark rearing rescues retinas expressing human P23H rhodopsin and is associated with a truncated mutant protein. A, B, Total rhodopsin levels from cyclic light or constant dark-reared primary transgenic animals (A) or F1 transgenic offspring (B) expressing human P23H rhodopsin. Dark-reared retinas on average contained more rhodopsin than their cyclic light-reared counterparts. F1 offspring segregated into two groups, which exhibited either a mild or severe RD phenotype. C, Confocal micrographs of retinal cryosections from F1 offspring from the severe phenotype group. The cyclic light-reared retinas showed greater loss of rods than did the dark-reared retinas. D, Immunolabeling of cryosections with mAbs 2B2 and 1D4. Regardless of light-rearing conditions, mAb 2B2 primarily labeled RIS, whereas mAb 1D4 labeling was significantly present in both RIS and ROS. In cyclic light-reared retinas, the labeling assumed a banded pattern, whereas in dark-reared retinas, continuous labeling along the length of the ROS was observed. WGA (red), Hoechst nuclear stain (blue), mAb 2B2, or 1D4 (green) are shown. gcl, Ganglion cell layer; n, nucleus; s, synapse; rpe, retinal pigment epithelium. Scale bars: C, 100 μm D, 5 μm. Image published in: Tam BM and Moritz OL (2007) Copyright © 2007. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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