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Figure 9. Lineage tracing of MyoD expressing cells in MyoDiCre/+ R26NG embryos, revealed by anti-GFP antibody (green) staining. (A–D) Lateral views of the right side of E10.5-E13.5 embryos; the dotted line indicates the sectional plane in (F–I). (Ei-iii) Lateral views of the left side of an E11.5 embryo, stained for Pax7 mRNA (blue) and GFP protein (green); dorsal to the top. (F) Frontal section of an E13.5 embryo, stained for Pax7 protein (red), GFP (green), and Dapi (blue). (G) Detail of the ventral rectus eye muscle, (H) detail of the tongue, (I) detail of the masseter as indicated in (D,F). The widely distributed bright green (F) or yellow cells (G–I) are autofluorescing blood cells. Cells with a history of MyoD expression can readily be detected at E10.5 and 11.5, first in the mandibular and hyoid arch, then in the developing extraocular muscles. In head-mesoderm-derived muscles, Pax7 mRNA and subsequent protein expression colocalises with that of MyoD-Cre driven GFP, and Pax7 containing nuclei reside in GFP expressing cells. In contrast, in the somite-derived tongue muscle, most Pax7-positive nuclei are not located in GFP expressing cells. Abbreviations as in Figures 1, 4, 5 and: eom, developing extraocular muscles. Image published in: Nogueira JM et al. (2015) Copyright © 2015 Meireles Nogueira, Hawrot, Sharpe, Noble, Wood, Jorge, Goldhamer, Kardon and Dietrich. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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