XB-IMG-144947
Xenbase Image ID: 144947
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Figure 1.
Whole-Embryo Phenotypes and Fibronectin Fibril Distribution in the Dorsal Axis
(A) Increasing severity of whole-embryo phenotype with amount of Xstbm overexpression.
(B and C) Increasing disruption of fibrillar fibronectin ECM organization with increasing amounts of Xstbm overexpression. (B) Projection of en face confocal sections (shown in schematic at left) shows increasing width of notochord field (asterisk) and uneven lateral boundaries with somitic mesoderm (arrowheads). (C) Projection of transverse sections (shown in schematic at left) shows that increasing amounts of Xstbm result in increasing amounts of fibril assembly throughout the notochord (asterisk) no longer restricted laterally by the somitic mesoderm (arrowheads). (D–F) Expression of dominant-negative Xstbm δPDZ-B and Xpk δLim-PET and overexpression of Xfz7 and Xpk produce severe whole-embryo phenotypes similar to severe Xstbm phenotypes (D), disrupt both planar (E) (en face projections of confocal z sections) and radial organization (F) (transverse projection of confocal z sections) of fibronectin ECM throughout the dorsal axial mesoderm (asterisk, notochord; arrowhead, lateral boundary of notochord and somitic mesoderm). Scalebars indicate 50 microns. Scale of (B) and (E) are the same, as are (C) and (F). ant, anterior; pos, posterior; dor, dorsal; ven, ventral; so, somitic mesoderm. Image published in: Goto T et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V.
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