XB-IMG-144958
Xenbase Image ID: 144958
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FIGURE 2. Expression of WIF-1 in the adult mouse and during Xenopus and zebrafish development.a, RNase protection assay using a mWIF-1 probe (top) or a mouse RNA polymerase II large-subunit probe (bottom); B, brain; E, eye; H; heart; K, kidney; Li, liver; Lu, lung; S, spleen; T, testis; Y, yeast tRNA. b–j, WIF-1 mRNAs detected by in situ hybridization. b–f, Xenopus; g–j, zebrafish. Expression is first detectable at the onset of somitogenesis in paraxial mesoderm (b, g). Expression is prominent in unsegmented presomitic mesoderm (psm), and is much weaker in newly formed somites (som; c, d, h, i). WIF-1 is expressed in the notochord (not) of 15-somite-stage zebrafish embryos (h) and stage-24 Xenopus embryos (d) in register with mature somites; no expression is detected in notochord regions flanked by unsegmented paraxial mesoderm. This pattern persists until the late stages of somitogenesis (e, i). In Xenopus tadpoles, strong WIF-1 expression is seen in visceral arches (va), and also in otic vesicles (ov), nasal placodes (npl) and several regions of the anterior brain (f). In the 24-h zebrafish embryo, expression is seen in the epiphysis (ep) and ventral midbrain (vmb) (j). Image published in: Hsieh JC et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd. Larger Image Printer Friendly View |