Xenbase Image ID: 144997

Fig. 5. Paraffin sections of embryos stained for expression of β- galactosidase, and processed by whole-mount in situ for expression of XcTnI mRNA. Sections shown are from stage 40 embryos, dorsal is at the top. 100 pg of mRNA encoding β-galactosidase was injected as a lineage tracer to indicate the injected side of the embryo. A white line is drawn through the midline of the sections, to emphasize the positioning of the heart. (A) Control embryo, coinjected with En control plasmid and mRNA encoding β-galactosidase. (B). Mutant embryo, coinjected with both dominant repressor Nkx constructs, and lineage tracer mRNA encoding β-galactosidase. Note the asymmetric placement of the XcTnI-positive myocardial the smaller size of the heart relative to that of the control (A). The small heart is formed on the side opposite to that of injection. M, midgut; N, notochord; O, otic vesicle and R, rhombencephalon Image published in: Fu Y et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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