Xenbase Image ID: 144998

Fig. 6. Whole-mount in situ analyses of embryos stained for expression of XNkx2-5, or XNkx2-5 and XcTnI. Embryos were injected with a total of 200 pg of DNA into one cell at the twocell stage, fixed, and processed for whole-mount in situ analyses staining for XNkx2-5 mRNA alone, or for both XNkx2-5 and XcTnI mRNAs utilizing double-label in situ (see Materials and Methods for details). XNkx2-5 mRNA signal was detected utilizing BCIP/NBT (brownish-purple color), and XcTnI mRNA signal was detected utilizing BCIP alone (light blue color). Overlap of the two signals gives a dark blue signal (E and F). Anterior is to the right. The mutant embryos shown were coinjected with both EnHDX2.3 and EnHDX2.5. (A) Control-injected stage 21 embryo, ventral view, showing symmetrical XNkx2-5 mRNA staining just posterior to the cement gland. (B) EnHD-injected stage 21 embryo, ventral view, with arrow indicating reduction in XNkx2-5 mRNA staining relative to the other side of the embryo. (C) Control injected stage 24 embryo, ventral view, showing XNkx2-5 mRNA in fused cardiac primordia posterior to the cement gland. (D). EnHD-injected stage 24 embryo, ventral view, with arrow indicating absence of XNkx2-5 mRNA staining on one side of the embryo. (E) Control injected stage 32 embryo, ventrolateral view, co-stained for XNkx2-5 and XcTnI mRNAs (dark blue). (F) EnHD-injected stage 33 embryo, ventrolateral view, co-stained for XNkx2-5 and XcTnI mRNAs. In this embryo, the positively staining regions have not yet fused. Although one side stains positively for both XNkx2-5 and XcTnI mRNAs (dark blue staining as indicated by blue arrowhead), the other side shows very faint staining for both mRNAs (black arrow). C, cement gland. Image published in: Fu Y et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 21 cardiac mesoderm nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 24 cardiac mesoderm heart primordium nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 32 heart heart primordium Image source: Published Larger Image Printer Friendly View

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