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Fig. 4. Expression of PTCRA transcripts. (A) PTCRA expression in hematopoietic tissues of E18, E14, and E10 chicken embryos was analyzed by RT-PCR. Three bands of 600 bp, 500 bp, and 400 bp, respectively, were identified in all of the stages. Bm, bone marrow; RT-, control PCR in which reverse transcriptase was omitted during first-strand synthesis; Sp, spleen; Th, thymus. S17 primers were used to assess the amount of cDNA samples used for PCR experiments. (B) (Left) Flow cytometric isolation of E18 chicken thymocyte subsets analyzed in Right. (Right) RT-PCR analysis of PTCRA expression on thymocyte subsets. PTCRA transcripts were detected in DN and DP thymocytes. S17 primers were used to assess the amount of cDNA samples used for PCR experiments. Image published in: Smelty P et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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