XB-IMG-145093
Xenbase Image ID: 145093
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Figure 1.
Anillin Localizes at Cell-Cell Junctions in Interphase and Mitotic Epithelial Cells
(A) Live imaging of Anillin-3XGFP in gastrula-stage embryos in which endogenous Anillin was depleted. mChe-membrane labels the plasma membrane. Images are brightest point projections of 17 apical z planes. Nuclear Anillin is not visible because only apical planes were captured. Right: z view shows that Anillin-3XGFP is apically focused. Graph shows an average of five line scans drawn perpendicular to junctions, indicating that the peak intensities of Anillin-3XGFP and mChe-membrane overlap.
(B) Embryos were injected with a GFP-membrane injection marker with or without the Anillin MO. Gastrula-stage embryos were fixed and stained with an anti-Anillin antibody (pseudocolored green), anti-GFP to view the membrane (pseudocolored red), and DAPI (blue). z views show that apically focused Anillin accumulation at cell-cell junctions is reduced when Anillin is depleted (yellow arrowheads).
(C) Quantification of the average intensity of endogenous Anillin at cell-cell junctions in control and Anillin KD cells (see Supplemental Experimental Procedures for details). Data are from three separate experiments; n = 18 embryos for control and n = 19 embryos for Anillin KD, graphed as box-and-whisker plot with the whiskers representing the 1st–99th percentile; p < 0.0001.
(D) Quantification of the average endogenous Anillin and DAPI intensity in the nucleus in control and Anillin KD cells (see Supplemental Experimental Procedures for details). Data are from three independent experiments; n = 18 embryos for control and n = 19 embryos for Anillin KD, graphed as box-and-whisker plot with the whiskers representing the 1st–99th percentile; p < 0.0001 for control versus Anillin KD for nuclear Anillin signal; p = 0.16 for control versus Anillin KD for DAPI signal.
See also Figure S1 and Movies S1 and S2. Image published in: Reyes CC et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |