Xenbase Image ID: 145094

Figure 2. AJs and TJs Are Disrupted when Anillin Is Knocked Down (A) Single intermediate plane views (top) and z views (bottom) of GFP-membrane in control and Anillin KD embryos reveal increased intercellular spaces in Anillin KD embryos (yellow arrows and arrowheads). (B–E) Fixed staining of control and Anillin KD embryos for β-catenin (B), E-Cadherin (C), ZO-1 (D), and Claudin (E). GFP-membrane or mChe-membrane was used as a MO injection marker, and DAPI labels DNA. z views show the normal localization of the cell-cell junction proteins in control cells as well as the disrupted localization in Anillin KD cells (see yellow arrowheads). The x-y TJ protein images on the left in (D) and (E) are maximal-intensity projections of serial z sections. The red arrow in (D) highlights an intercellular space between a dividing cell and its neighbor, whereas the yellow arrow indicates a ZO-1 concentration that is buried basally. (F) Quantification of β-catenin polarization in control and Anillin KD cells from line scans along the basolateral surface. The β-catenin signal at the ten most-basal points was normalized to zero so that data from multiple embryos could be averaged (see Supplemental Experimental Procedures for details). Data are from two independent experiments; n = 26 embryos for control and n = 18 embryos for Anillin KD, graphed as mean ± SEM; ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001. (G) Quantification of the relative intensity of Claudin at cell-cell junctions by generating line scans perpendicular to junctions (see Supplemental Experimental Procedures for details). Data are from two independent experiments; n = 10 embryos for control and n = 12 for Anillin KD, graphed as mean ± SEM; p < 0.0001. See also Figure S2. Image published in: Reyes CC et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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