Xenbase Image ID: 145095

Figure 3. Junctional Integrity Is Compromised when Anillin Is Knocked Down (A) Live control or Anillin KD embryos were mounted in 3,000 molecular weight (MW) Alexa 488-Dextran. Left: x-y views show that dextran can penetrate between rounded cells in Anillin KD embryos. Right: z views generated along the indicated lines (top) and 3D views (bottom) show that, although dextran remains at the surface in control embryos, it can penetrate between cells in Anillin KD embryos. (B) Quantification of the average percentage of junctions where dextran penetrated into the intercellular space in control and Anillin KD embryos. Data are from three independent experiments; n = 13 embryos for controls and n = 17 embryos for Anillin KD, graphed as mean + SEM; p < 0.0001. (C) Quantification of the average depth of dextran penetration for control and Anillin KD embryos. Data are from three independent experiments; n = 13 embryos for controls and n = 17 embryos for Anillin KD, graphed as box-and-whisker plot with the whiskers representing the 1st–99th percentile; p < 0.0001. See also Figure S2. Image published in: Reyes CC et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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