Xenbase Image ID: 145100

Figure S3. Anillin regulates the distribution of Rho-GTP, F-actin, and P-MLC. E. E. Frames from live confocal movies of F-actin in control and Anillin OE embryos. Embryos were injected with 20 ng/µl Anillin mRNA and imaged by live confocal microscopy at late blastula (stage 9). Embryos were co-injected with a probe for F-actin (mChe-UtrCH). Yellow arrows highlight dividing cells, and the red-boxed regions are enlarged on the right. When Anillin is OE, the F-actin in the contractile ring is intense and spiky, and the cells divide more slowly. F. (Left) merged images that correspond to those in Figure 4F of F-actin (red), GFPmem (green), and nuclei (blue) for control, Anillin KD, and Anillin OE embryos. For OE, embryos were injected with 40 ng/µl Anillin mRNA. (Right) z views of F-actin staining for control, Anillin KD, and Anillin OE embryos. F-actin is concentrated at the apical surface of each junction in controls (yellow arrowheads). However, F-actin accumulation is reduced in apically domed Anillin KD cells. In Anillin OE embryos, F-actin is more strongly accumulated at junctions and the apical cell cortex (yellow arrow) compared with controls. G. (Left) merged images that correspond to those in Figure 4G of P-MLC (red), GFPmem (green), and nuclei (blue) for control, Anillin KD, and Anillin OE embryos. For OE, embryos were injected with 40 ng/µl Anillin mRNA. (Right) z views of P-MLC for control, Anillin KD, and Anillin OE embryos. P-MLC is accumulated at the apical surface of each junction in controls (yellow arrowheads). However, P-MLC accumulation is reduced or lacking in Anillin KD cells. The remaining P-MLC signal in Anillin KD cells often is more broadly distributed compared with the focused apical signal in controls. In Anillin OE embryos, P-MLC is more strongly accumulated at junctions and the apical cell cortex (yellow arrows) compared with controls, and the apical cell surface is sometimes contracted (left yellow arrow). See also Movies S3-S5. Image published in: Reyes CC et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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