Xenbase Image ID: 145156

Figure 1. Injection of an Oligo Antisense to VegT mRNA Depletes the Endogenous Maternal VegT mRNA, but Does Not Prevent the Transcription of Zygotic VegT (A) Northern blot, probed for VegT RNA from embryos derived from one batch of oocytes frozen at the stages indicated. O, 4 ng VT9M oligo injected; U, uninjected. The blot was stripped and reprobed for Xbra and EF1α. The arrowhead points to residual VegT signal that was incompletely stripped before adding Xbra probe. (B) Northern blot of sibling embryos to those in Figure 1A that were injected with oligo VT9M at the same time as those in Figure 1A and, 24 hr later, were also injected with synthetic VegT mRNA (300 pg) before fertilization (O PLUSPUSSIGN R). The injected mRNA is indicated by the arrow. The appearance of sibling gastrulae treated in the same fashion from this experiment is shown in Figure 5A. Image published in: Zhang J et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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