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Figure 7. Maternal VegT Expression Is Required for Mesoderm-Inducing Signals to Be Released by Vegetal Cells (A–D) Nieuwkoop recombinants cultured to the early neurula stage. (A) Uninjected animal cap/ uninjected vegetal mass. (B) VegT-depleted animal cap/uninjected vegetal mass. (C) Uninjected animal cap/VegT-depleted vegetal mass. (D) VegT-depleted animal cap/VegT-depleted vegetal mass. (E) Animal caps cultured on vegetal masses for 2 hr and then separated and cultured to sibling early tailbud stage. These caps were analyzed by RT-PCR in (F). Upper left are uninjected caps that were cultured with wild-type bases for 2 hr (wt/wt). Upper right are animal caps from VegT-depleted embryos cultured with wild-type bases (VgT/wt). Lower left are wild-type caps cultured with VegT-depleted bases (wt/VgT), and lower right are VgT-depleted caps cultured with VegT-depleted bases (VgT/VgT). (F) RT-PCR analysis on animal caps shown in (E) for mesodermal and epidermal markers. Image published in: Zhang J et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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