Xenbase Image ID: 145164

Fig. 1. N-tubulin is RA responsive, and requires RARα and RARγ for its expression; neural progenitor markers Geminin and Foxd4l1 are modulated by RAR signaling. (A-C,G-I,M-O) Whole-mount in situ hybridization of N-tubulin expression from whole embryos treated at stage 7/8 with 1 μM TTNPB (an RAR-specific agonist), 1 μM AGN193109 (an RAR-specific antagonist) or control vehicle (0.1% ethanol). (A,G,M) Control expression of N-Tubulin, Geminin and Foxd4l1. (B,C) Overexpression of neurons was observed in 15/19 of TTNPB-treated embryos (B); loss of neurons was observed in 19/19 of AGN193109-treated embryos (C). (H,N) Geminin (9/9 embryos) and Foxd4l1 (9/10) expression was diminished with TTNPB. (I,O) AGN193109 expanded Geminin (9/9) and Foxd4l1 (14/14). (D-F,J-L,P-R) All embryos were injected unilaterally at the two- or four-cell stage. The injected side is indicated by the magenta β-gal mRNA lineage tracer. (D,J,P) Control expression of N-tubulin, Geminin and Foxd4l1. (E,K,Q) 3.3 ng Rarα1 MO + 3.3 ng Rarα2.1 MO + 3.3 ng Rarα2.2 MO reduced expression of N-tubulin (18/23 embryos), but expanded expression of Geminin (13/13) and Foxd4l1 (14/20). (F,L,R) 3.75 ng Rarγ1 MO + 3.75 Rarγ2 MO reduced expression of N-tubulin (34/34), but expanded expression of Geminin (35/35) and Foxd4l1 (14/16). Embryos are shown in dorsal view at stage 14; anterior is at the bottom. Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 14 neuron Rohon-Beard neuron neural progenitor cell gmnn.L gem, geminin X. laevis Throughout NF stage 14 neural plate neural progenitor cell foxd4l1.1.S foxd4, Foxd4l1, foxd4l1.1-a, foxd4l1.1-b, foxd5, foxD5a, FoxD5b, Xfd-12, XFlip, xlFoxD5a, xlFoxD5b, xtFoxD5 X. laevis Throughout NF stage 14 neural plate neural progenitor cell Image source: Published Larger Image Printer Friendly View

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