XB-IMG-145165
Xenbase Image ID: 145165
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Fig. 2.
RAR signaling modulates Erf and Etv3l expression. (A) QPCR showing Erf, Etv3 and Etv3l expression in embryos treated at stage 7/8 with 1 μM TTNPB, 1 μM AGN193109 or vehicle (0.1% ethanol). The y-axis represents 2-δδCt values normalized to Histone H4 and expressed as fold induction relative to control. Erf is induced by TTNPB, Etv3l is induced by TTNPB in early neurula, whereas Etv3 is not. Asterisks represent statistical significance compared with control (*=P≤0.05, **=P≤0.01). (B-D) Whole-mount in situ hybridization of Erf expression from whole embryos treated at stage 7/8 with 1 μM TTNPB, 1 μM AGN193109 or vehicle (0.1% ethanol). Expansion of Erf was observed in 13/13 of TTNPB-treated embryos; blurring of Erf expression in the neural folds was observed in 13/13 of AGN193109-treated embryos. (E-G) Embryos were injected unilaterally at the two- or four-cell stage. The injected side is indicated by the magenta lineage tracer. (E) Control expression of Erf. (F) 3.3 ng Rarα1 MO + 3.3 ng Rarα2.1 MO + 3.3 ng Rarα2.2 MO reduced expression of Erf (10/10). (G) 3.75 ng Rarγ1 MO + 3.75 Rarγ2 MO reduced expression of Erf (11/11). Embryos are shown in dorsal view at stage 14; anterior is at the bottom. Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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