XB-IMG-145168
Xenbase Image ID: 145168
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Fig. 5.
ERF or ETV3/3L knockdown expands expression of Zic1, Zic2, Zic3, Geminin and Foxd4l1 in the anterior/dorsal region. Embryos were injected unilaterally at the two- or four-cell stage. The injected side is indicated by the magenta lineage tracer. (A,D,G,J,M) Control expression of Zic1, Zic2, Zic3, Geminin and Foxd4l1. (B,E,H,K,N) Embryos injected with 10 ng Erf MO showed lateral expansion (13/14) and posterior reduction (13/14, green arrow) of Zic1; stripe blurring (23/23) and posterior reduction (10/23) of Zic2; stripe blurring (14/21) and posterior reduction (6/21) of Zic3; and expansion of Foxd4l1 (10/16) and Geminin (16/22). (C,F,I,L,O) Embryos injected with 20 ng Etv3/3l MO showed lateral expansion (12/15) and posterior reduction (12/15, green arrow) of Zic1; stripe blurring (14/14), lateral expansion (5/14) and posterior reduction (10/14) of Zic2; stripe blurring (14/14), lateral expansion (18/21) and posterior reduction (8/21) of Zic3; and expansion of Foxd4l1 (16/20) and Geminin (16/16). Embryos are shown in dorsal view at stage 14; anterior is at the bottom. Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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