XB-IMG-145170
Xenbase Image ID: 145170
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Fig. 7.
ERF or ETV3L knockdown increases proliferation in the neural plate. Embryos were injected unilaterally at the 2- or 4-cell stage. The injected side is indicated by the red fluorescent lineage tracer. Representative photographs in bright-field (A,D,G) and fluorescence (B,E,H) are shown. Quantitation of phospho-Histone H3 nuclei staining (scored blindly within dotted line ovals and normalized to account for differences in staining intensity) for all embryos in the experiment is provided in bar graphs (C,F,I). Embryos are shown in dorsal view at stage 14; anterior is at the bottom. (A-C) Embryos injected with 10 ng control MO showed no significant difference in the number of phospho-Histone H3 nuclei on the injected versus the uninjected side (n=27; P=0.71). (D-F) Embryos injected with 10 ng Erf MO showed an increased number of phospho-Histone H3 nuclei on the injected side (n=23; *P=0.022). (G-I) Embryos injected with 10 ng Etv3l MO showed an increased number of phospho-Histone H3 nuclei on the injected side (n=26; ***P=0.0009). Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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