Xenbase Image ID: 145171

Fig. 8. ERF gain of function leads to precocious neurogenesis under appropriate conditions. (A-L) All embryos were injected unilaterally with 0.1 ng hGR-Erf (S246,251 A246,251) mRNA at the two- or four-cell stage, then treated with 10 μM dexamethasone (DEX) or 0.1% DMSO vehicle at various stages. The injected side is indicated by the magenta β-gal mRNA lineage tracer. (A,D,G,J) DMSO-treated embryos. (B) Dexamethasone treatment at stage 9 produced reduction of N-tubulin in 40% of embryos. (E) Dexamethasone treatment at stage 10.5 produced precocious neurogenesis in 28% of embryos. (H) Dexamethasone treatment at stage 11 produced precocious neurogenesis in 27% of embryos. (K) Embryos treated with dexamethasone at stage 11.5 were mostly unchanged. The red arrows indicate the increase in N-tubulin expression. (C,F,I,L) Scoring of embryos in A-K: LT, low tubulin; PMN, premature neurogenesis; ESN, extra sensory neurons; NC, no change. All embryos are shown in dorsal view with anterior at the bottom, at stage 13/13.5. Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 14 neuron Image source: Published Larger Image Printer Friendly View

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