Xenbase Image ID: 145174

Fig. S2. Dose response of TTNPB and AGN193109. (A,B) Whole-mount in situ hybridization from whole embryos treated at stage 7/8 with 1 nM to 1 μM TTNPB, 10 nM to 1 μM AGN193109 or control vehicle (0.1% ethanol). Dorsal N-tubulin expression is increased with TTNPB treatment at doses as low as 10–9 M, compared with control. AGN193109 causes loss of N-tubulin expression at doses as low as 10–7 M. (C) QPCR showing HoxA1 expression in embryos treated at stage 7/8 with 1 nM to 1 μM TTNPB, 1 nM to 1 μM AGN193109 or vehicle (0.1% ethanol). The y-axis represents 2–δδCt values normalized to Histone H4 and expressed as fold induction relative to control. HoxA1 is induced by TTNPB at doses as low as 10–9 M and repressed by AGN193109 at doses as low as 10–8 M. Asterisks represent statistical significance compared with control (*P≤0.05, **P≤0.01, ***P≤0.001). Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 14 neuron Image source: Published Larger Image Printer Friendly View

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