Xenbase Image ID: 145178

Fig. S6. Sox3 is modulated by RAR signaling; ERF or ETV3/3L knockdown expand expression of Sox3. (A-C) Whole-mount in situ hybridization from whole embryos treated at stage 7/8 with 1 μM TTNPB, 1 μM AGN193109 or control vehicle (0.1% ethanol). Sox3 expression is slightly narrowed in the anterior domain with TTNPB treatment (17/19) compared with control. AGN193109 (a RAR-specific antagonist) causes anterior expansion of Sox3 (15/22). (D-I) All embryos were injected unilaterally at the two- or four-cell stage. The injected side is indicated by the magenta β-gal mRNA lineage tracer. (D,G) Control expression of Sox3. (E) 3.3 ng Rarα1 MO + 3.3 ng Rarα2.1 MO + 3.3 ng Rarα2.2 MO expanded expression of Sox3 (13/15 embryos). (F) 3.75 ng Rarγ1 MO + 3.75 Rarγ2 MO expanded expression of Sox3 (21/22). (H,I) 10 ng Erf MO expanded Sox3 expression (8/14) and 20 ng Etv3/3l MO expanded Sox3 expression (11/17). All embryos are shown in dorsal view with anterior at the bottom, at stage 14. Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox3.L Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 14 neural plate Image source: Published Larger Image Printer Friendly View

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