Xenbase Image ID: 145180

Fig. S8. ERF or ETV3L knockdown rescues the extra/ectopic neuron phenotype generated by VP16-Rarα/γ mRNA. All embryos were injected unilaterally at the two- or four-cell stage. The injected side is indicated by the magenta β-gal mRNA lineage tracer. (A,C) 10 ng or 20 ng control MO + 0.1 ng mCherry (control) mRNA does not change expression of N-tubulin. (B,D) 10 ng or 20 ng control MO + 0.1 ng VP16- Rarα/γ mRNA results in extra and/or ectopic neurons. (E-H) 10 ng Erf MO partially rescues (68%) and 20 ng Erf MO completely rescues (97%) VP16-Rarα/γ mRNA extra/ectopic neuron phenotype. (I-L) 10 ng or 20 ng Etv3l MO completely rescues (100%) VP16-Rarα/γ mRNA extra/ectopic neuron phenotype. Embryos are shown in dorsal view with anterior at the bottom, at stage 14. (M) Detailed scoring of embryos represented in A-L. Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 14 neuron Image source: Published Larger Image Printer Friendly View

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