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XB-IMG-145183

Xenbase Image ID: 145183

Fig. S11. ERF or ETV3L knockdown does not change proliferation outside of the neural plate. Embryos were injected unilaterally at the 2- or 4-cell stage. The injected side is indicated by the red fluorescent lineage tracer. (A-D,F-I,K-N) Representative photographs in bright-field (A,B,F,G,K,L) and fluorescence (C,D,H,I,M,N) are shown. (A-D) Photographs of the same stage 14 embryo, in lateral view, with B and D flipped horizontally from A and C, such that the anterior of the embryo is always on the left. The same is true for F-I and K-N. (E,J,O) Quantitation of phospho-Histone H3 staining for all embryos in the experiment is provided in bar graphs. (A-O) Embryos injected with 10 ng control MO (A-E), 10 ng Erf MO (F-J) or 20 ng Etv3l MO (K-O) showed no significant difference in phospho-Histone H3 staining on the injected versus the uninjected side (Ctrl MO, n=21, P=0.4654; Erf MO, n=39, P=0.5329; Etv3l MO, n=47, P=0.2256).

Image published in: Janesick A et al. (2013)

Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
h3-3a.Lh3.3, h3.3a, h3f3aX. laevisThroughout NF stage 14mitotic cell

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