Xenbase Image ID: 145185

Fig. S13. Subcellular localization of hGR-ERF. COS-7 cells were co-transfected with mCherry and either Erf (no FLAG), FLAGhGR- Erf (WT), FLAG-hGR-Erf (S246,251→A246,251) or FLAG-hGR-Erf (S246,251→E246,251), then treated with 1 μM dexamethasone (DEX) or 0.01% DMSO. (A,B) Negative control with Erf (no FLAG); transfected cells are indicated by mCHERRY fluorescence. (C,E,G) All FLAG-hGR-ERF proteins are found mostly in the cytoplasm when cells were treated with DMSO. (D) Dexamethasone-treated FLAG-hGR-ERF (WT) is located in the cytoplasm and the nucleus. (F) Dexamethasone-treated FLAG-hGR-ERF (S246,251→A246,251) is located exclusively in the nucleus (H) Dexamethasone-treated FLAG-hGR-ERF (S246,251→E246,251) is located in the cytoplasm and the nucleus. Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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