XB-IMG-145186
Xenbase Image ID: 145186
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Fig. S14. ERF gain of function increases primary neurons. Embryos were injected unilaterally at the 2- or 4-cell stage, then treated
with 10 μM dexamethasone (DEX) or 0.1% DMSO vehicle at stage 11. Quantitation of N-tubulin expression at stage 14 is shown.
(A) Embryos injected with 0.1 ng or 25 pg hGR-Erf (S246,251→A246,251) mRNA and treated with dexamethasone showed a significant
increase in neurons on the injected side (0.1 ng, n=29, P=0.0073; 25 pg, n=23, P=0.0049). DMSO-treated embryos showed no
significant difference in neurons (0.1 ng, n=22, P=0.4170; 25 pg, n=22, P=0.2558) (B) Embryos injected with 0.1 ng or 25 pg hGR-Erf
(S246,251 → E246,251) mRNA showed no significant difference in neurons on the injected versus the uninjected side in dexamethasonetreated
(0.1 ng, n=22, P=0.2055; 25 pg, n=13, P=0.7354) or DMSO-treated embryos (0.1 ng, n=16, P=0.6233; 25 pg, n=10,
P=0.1309). Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |