Xenbase Image ID: 145189

Fig. S17. ERF gain of function decreases proliferation in the neural plate. Embryos were injected unilaterally at the 2- or 4-cell stage, then treated with 10 μM dexamethasone (DEX) or 0.1% DMSO vehicle at stage 11. The injected side is indicated by the red fluorescent lineage tracer. (A,B,D,E) Representative photographs in bright field (A,D) and fluorescence (B,E) are shown. (C,F) Quantitation of phospho-Histone H3 staining for all embryos in the experiment. Embryos are shown in dorsal view at stage 14; anterior is at the bottom. (A-C) DMSO-treated embryos injected with 0.1 ng hGR-Erf (S246,251→A246,251) mRNA showed no significant difference in the number of phospho-Histone H3 nuclei on the injected versus the uninjected side (n=30; P=0.758). (D-F) Dexamethasone-treated embryos injected with 0.1 ng hGR-Erf (S246,251→A246,251) mRNA showed a decreased number of phospho- Histone H3 nuclei on the injected versus the uninjected side (n=37; P=0.0028). Image published in: Janesick A et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue h3-3a.L h3.3, h3.3a, h3f3a X. laevis Throughout NF stage 14 mitotic cell Image source: Published Larger Image Printer Friendly View

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