Xenbase Image ID: 145271

Fig. 1 NeuroD4 is expressed and phosphorylated during primary neurogenesis (A-C). a Protein sequence alignment for human, mouse and Xenopus NeuroD4 homologues using Clustal W. The conserved bHLH domain is indicated in green and SP/TP sites are highlighted in red. A consensus line is also shown below the alignment to indicate the degree of conservation of amino acids at each position: (*) denotes identical residues in all three sequences; (:) denotes highly conserved residues; (.) denotes weakly conserved residues. b Endogenous expression of NeuroD4 at stage 14 (i) and neural-β-tubulin at stage 17 (ii) was determined by whole mount ISH (white arrows correspond to zones of primary neurogenesis; TG = trigeminal ganglia). Dorso-ventral views, anterior up, stages as indicated. c Western Blot analysis of extracts from stage 13 embryos injected with an HA-tagged wild type (WT) NeuroD4 mRNA, either with or without protein phosphatase treatment. Tubulin was used as a loading control Image published in: Hardwick LJ and Philpott A (2015) © Hardwick and Philpott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue neurod4.L ath-3, ath3, atoh3, atonal homolog-3, bhlha4, math-3, Xath-3, Xath3 X. laevis Throughout NF stage 14 neuroectoderm neural plate neural plate border neural progenitor cell tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 17 neural plate pre-chordal neural plate chordal neural plate Rohon-Beard neuron neurogenic placode neuroectoderm trigeminal placode Image source: Published Larger Image Printer Friendly View

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