Xenbase Image ID: 145272

Fig. 2 Phospho-mutant 6T/S-A NeuroD4 has increased proneural activity relative to WT NeuroD4 (a-g). a Schematic representation of WT NeuroD4 and full phospho-mutant 6 T/S- A NeuroD4 protein sequences, indicating the relative positions of the six SP or TP sites that have been mutated to AP sites. b-g Two-cell stage embryos were unilaterally injected with 100 pg of either WT or 6T/S-A NeuroD4 mRNA, and at stage 18, gene expression was assayed by qRT-PCR (b), or by whole mount ISH (d-g) with representative embryo images shown in (c). For qRT-PCR analysis (b) significance is calculated as described in the methods section for phospho-mutant NeuroD4 relative to WT NeuroD4 (blue adjoining lines and stars) and relative to uninjected control embryos (shown with black stars) [n = 5]; (p <0.05) = *; (p <0.025) = **; (p <0.0125) = ***. For ISH analysis, embryos were scored according to the scale described in Additional file 1 for expression of neural-β-tubulin (D [n = 100–115]), p27Xic1 (E [n = 24-33]), xMyt1 (F [n = 26-32]), and xNeuroD1 (G [n = 23- 28]). Views in (C [i-iii]) are dorso-ventral (DV); views in (C [iv]) are rostro-caudal (RC) with dorsal surface facing up, trigeminal ganglia indicated by arrows. All images show injected side to the right. Image published in: Hardwick LJ and Philpott A (2015) © Hardwick and Philpott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Sometime during NF stage 17 to NF stage 18 trigeminal placode neuroectoderm neural plate Rohon-Beard neuron neural progenitor cell cdknx.L p27Xic-1, p27XIC1, p28, Xic-1, Xic1 X. laevis Sometime during NF stage 17 to NF stage 18 trigeminal placode neuroectoderm neural plate neural progenitor cell myt1.S X-MyT1, xmyt1, XMyTI X. laevis Sometime during NF stage 17 to NF stage 18 trigeminal placode neuroectoderm neural plate neural progenitor cell neurod1.L neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. laevis Sometime during NF stage 17 to NF stage 18 trigeminal placode neuroectoderm neural plate neural progenitor cell Image source: Published Larger Image Printer Friendly View

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