Xenbase Image ID: 145275

Fig. 5 Cumulative mutation of phosphorylation sites creates step-wise increases in proneural activity (a-d). a Schematic representation of the phospho-mutant series of constructs demonstrating the SP and/or TP sites that are mutated to AP sites in each. The series consists of cumulative mutation of sites, working from N to C termini. Two-cell stage embryos were unilaterally injected with 100 pg mRNA of the respective NeuroD4 construct and assayed at stage 18 for expression of neural-β-tubulin by qRT-PCR (B[n = 3]), or whole embryo ISH (C[n = 59-77]) with representative images shown in (d). Views are dorso-ventral with injected side to the right; (p <0.05) = *; (p <0.025) = **; (p <0.0125) = *** Image published in: Hardwick LJ and Philpott A (2015) © Hardwick and Philpott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 18 trigeminal placode neuroectoderm neural plate Rohon-Beard neuron neural progenitor cell Image source: Published Larger Image Printer Friendly View

Return to previous page