Xenbase Image ID: 145281

Fig. 2. Shh inverts its action on PKA activity during spinal cord development. (A) Neural plates and spinal cords were incubated for 30 min with indicated agents and processed for PKA activity measurements with a nonradioactive PKA assay. Images are representative examples of the PKA activity assay. Graph shows mean ± SEM PKA activity (P-substrate/non–P-substrate optical density ratio) for the indicated treatments; n ≥ 5, *P < 0.05, **P < 0.001, ***P < 5E-5 compared with control (incubated with vehicle only); ns: not significant; verat: veratridine. (B) Dissociated neural plate and spinal cord cells from AKAR2-CR–expressing 14.25- and 21-hpf embryos were time-lapse imaged every 30 s. (Left) Representative ratiometric (acceptor-mRuby/donor-Clover) images of cells before and 30 min after addition of 10 nM Shh. Grayscale bar represents acceptor/donor ratio increasing from black to white. Traces represent mean ± SEM percentage change in emission ratio; n ≥ 29 cells per condition. (Scale bar: 20 μm.) Image published in: Belgacem YH and Borodinsky LN (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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