XB-IMG-145283
Xenbase Image ID: 145283
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Fig. 4. Shh activates CREB that represses gli1 transcription in the developing spinal cord. (A) Shown is a representative Western blot assay from spinal cord samples incubated in the absence or presence of 100 nM SAG for 40 min and immunoprobed for activated CREB (Ser133-P-CREB) and H2B as loading control. (B) Schematic of luciferase reporters used to assess regulation of gli1 transcription. Sequences of three identified CREs and their relative positions to the ATG of the human gli1 first untranslated exon. (C) Neural plates and spinal cords from embryos expressing wt-reg-hgli1-luciferase or mut-reg-hgli1-luciferase in the absence or presence of CREB overexpression were incubated with indicated agents or vehicle for 8 h and processed for luciferase activity measurements. Graph shows mean ± SEM normalized luciferase intensity; n ≥ 5; *P < 0.05, **P < 0.005, and ***P < 0.00005; ns: not significant. Image published in: Belgacem YH and Borodinsky LN (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |